The Human Polycystic Kidney Disease RT² Profiler PCR Array profiles the expression of 84 key genes involved in growth of renal cysts, which often lead to end-stage renal disease. Polycystic kidney diseases (PKDs) represent a large group of progressive renal disorders characterized by cystic expansion of the kidneys resulting in progressive kidney enlargement and renal insufficiency. The most common PKDs are inherited as either autosomal dominant or autosomal recessive traits. Studies of autosomal dominant and recessive PKDs converge on molecular mechanisms of cystogenesis, including ciliary abnormalities and intracellular calcium dysregulation that ultimately lead to increased proliferation, apoptosis, and dedifferentiation. Recent advances in understanding the role of signaling molecules, (such as cyclic AMP, calcium, integrins, and bone morphogenetic proteins), as well as angiogenic, differentiation, and mitogenic factors in renal cystogenesis and dysfunction have led to intriguing possibilities for therapeutic intervention. The genes profiled with this array are associated with angiogenic, mitogenic, and inflammatory responses and factors responsible for calcium signaling, primary cilia function, and transcriptional regulation among others. A set of controls present on each array enables data analysis using the ΔΔCT method of relative quantification and assessment of reverse transcription performance, genomic DNA contamination, and PCR performance. Using real-time PCR, research studies can easily and reliably analyze the expression of a focused panel of genes involved in polycystic kidney disease with this array.
Angiogenic Factors: EDN1, NRP2, TPM1, VEGFA, IL8.
Apoptosis: BCL2L1, DAPL1.
BMP/TGFb/Activin Signaling: BMP2, BMP4, BMP7, GREM1, NOG, WNT11.
Calcium Signaling: PKD1, PKD2, SLC26A7, SLC2A9, SLC5A10.
cAMP Signaling: ACY1, ADCY2, ADCY3, PTGER2, PTGER3, PTGS2, ADCY7, CREBBP, P2RX7.
Nephron Segment Differentiation: ANGPT2, APOE, APOM.
Integrin Signaling: CLDN10, CLU, COL12A1, COL3A1, FBLN1, FN1, PCDH7, RHOA, ITGA8, SPRY1.
Growth Factor-Receptor Interaction: EGR3, FGF1, FGFR3, IGF1R, NTRK3, TGFB3.
Immune/Inflammatory Pathways: CFD, CXCL1, CYP8B1, IL17D, IL6, KNG1, NRG1, PCK1, PRLR, PTPRZ1, IL8.
mTOR pathway: PIK3CA, PIK3R1.
Primary Cilia: CYS1, DNAH5, IFT88, LGALS3, PDGFRA, PKHD1, TMEM27, UMOD, GLI2.
Transcription Factors: FOSB, JUN, GLI2, WT1.
Other Genes: AGXT2, ALDH8A1, CLCNKA, DPEP1, HGD, HRG, HSD11B2, MIOX, NAPSA, OSR2, PRKCB, RET, SCN5A, VGLL3